Cellino Biotech, Inc.

Systems and methods are disclosed for enabling optical bioprocesses in cell manufacturing. A cell manufacturing platform comprises a cell culture cassette supporting a cell culture; an optical engine configured to: capture images of the cell culture; and optically remove cells from the cell culture; and wherein the cell culture cassette remains stationary and the optical engine moves relative to the cell culture cassette to capture the images and to remove cells.

Systems and methods for cell culturing are disclosed herein. A fluidic exchange system for cell culturing comprises a cell culture cassette, comprising: a cell culture chamber having a cell culture and fluid media; and one or more pluggable ports fluidically connected to the cell culture chamber; a fluid media cassette, comprising: one or more liquid storage compartments; and one or more pluggable ports fluidically connected to the one or more liquid storage compartments; and a cassette coupler configured to fluidically couple the cell culture cassette and the fluid media cassette.

A method for cell culture expansion and maintenance, comprising seeding, in a first cell culture cassette, a plurality of cell colonies adhered to a first surface of the first cell culture cassette; capturing, by an optical engine, time-series images of the plurality of cell colonies as the plurality of cell colonies expand; determining, by a platform manager, a subset of cell colonies to remove from the first surface based on the time-series images; removing, by the optical engine, the subset of cell colonies from the first surface; washing, by a fluid management system, the removed subset of cell colonies from the first cell culture cassette; repeating steps for one or more iterations; harvesting a set of remaining cell colonies from the first cell culture cassette; seeding the set of remaining cell colonies into a second cell culture cassette; repeating steps for additional iterations in the second cell culture cassette.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

The disclosure relates to growing cells, directing cells to grow into specified cell types, genetically and physically manipulating cells, and addressing one or more individual cells within a mixed cell population. Aspects of the disclosure relate to vectors useful to induce developmental changes in cells, in which those vectors have a temporal component. Vectors of the disclosure encode a controllable, temporal series of events. Once the vectors are delivered into target cells, a series of discrete and different genetic events may be induced. The disclosed methods generally provide for the temporal encoding of multiplex genetic effectors in vector format for cell state transitions.

The disclosure relates to growing cells, directing cells to grow into specified cell types, genetically and physically manipulating cells, and addressing one or more individual cells within a mixed cell population. Aspects of the disclosure relate to vectors useful to induce developmental changes in cells, in which those vectors have a temporal component. Vectors of the disclosure encode a controllable, temporal series of events. Once the vectors are delivered into target cells, a series of discrete and different genetic events may be induced. The disclosed methods generally provide for the temporal encoding of multiplex genetic effectors in vector format for cell state transitions.

Methods, systems, and computer program products are provided for monitoring a cell culture, utilizing a predictive model to improve cell culture processes, measuring cell dynamics of a cell culture, managing cell clusters in a cell culture system, and training a machine learning model for managing cell clusters in a cell culture system. In various embodiments, a cell culture may be maintained in a cell culture chamber. The cell culture may comprise one or more cell colonies. One or more properties of the cell culture may be measured. One or more perturbations may be applied to all or a portion of the cell culture. The one or more properties of the cell culture may be measured after applying the one or more perturbations. The one or more properties may be compared before and after the one or more perturbations. The cell culture may be managed based on the comparison.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.

Disclosed herein are platforms, systems, and methods including a cell culture system that includes a cell culture container comprising a cell culture, the cell culture receiving input cells, a cell imaging subsystem configured to acquire images of the cell culture, a computing subsystem configured to perform a cell culture process on the cell culture according to the images acquired by the cell imaging subsystem, and a cell editing subsystem configured to edit the cell culture to produce output cell products according to the cell culture process.