DAESANG CORPORATION

The present invention relates to a Corynebacterium sp. mutant microorganism producing L-glutamic acid and a method of producing L-glutamic acid using the same, and more specifically, to a novel nicotinamide mononucleotide transporter variant involved in the L-glutamic acid biosynthetic pathway, a polynucleotide, and a transformant, as well as a method of producing L-glutamic acid using the same. The nicotinamide mononucleotide transporter variant according to the present invention is obtained by substituting one or more amino acids in the amino acid sequence constituting nicotinamide mononucleotide transporter to change the enzymatic activity of the nicotinamide mononucleotide transporter, and a recombinant microorganism comprising the nicotinamide mononucleotide transporter variant is capable of efficiently producing L-glutamic acid.

The present invention relates to a Corynebacterium sp. mutant microorganism producing L-glutamic acid and a method of producing L-glutamic acid using the same, and more specifically, to a novel oxidoreductase variant involved in the L-glutamic acid biosynthetic pathway, a polynucleotide, and a transformant, as well as a method of producing L-glutamic acid using the same. The oxidoreductase variant according to the present invention is obtained by substituting one or more amino acids in the amino acid sequence constituting oxidoreductase to change the enzymatic activity of the oxidoreductase, and a recombinant microorganism comprising the oxidoreductase variant is capable of efficiently producing L-glutamic acid.

The present invention relates to a Corynebacterium sp. mutant microorganism producing L-glutamic acid and a method of producing L-glutamic acid using the same, and more specifically, to a novel NADP-dependent malic enzyme variant involved in the L-glutamic acid biosynthetic pathway, a polynucleotide, and a transformant, as well as a method of producing L-glutamic acid using the same. The NADP-dependent malic enzyme variant according to the present invention is obtained by substituting one or more amino acids in the amino acid sequence constituting NADP-dependent malic enzyme to change the enzymatic activity of the NADP-dependent malic enzyme, and a recombinant microorganism comprising the NADP-dependent malic enzyme variant is capable of efficiently producing L-glutamic acid.

The present invention relates to a Corynebacterium sp. mutant microorganism producing L-glutamic acid and a method of producing L-glutamic acid using the same, and more specifically, to a novel biotin-protein ligase variant involved in the L-glutamic acid biosynthetic pathway, a polynucleotide, and a transformant, as well as a method of producing L-glutamic acid using the same. The biotin-protein ligase variant according to the present invention is obtained by substituting one or more amino acids in the amino acid sequence constituting biotin-protein ligase to change the enzymatic activity of the biotin-protein ligase, and a recombinant microorganism comprising the biotin-protein ligase variant is capable of efficiently producing L-glutamic acid.

The present invention relates to a Corynebacterium sp. mutant microorganism producing L-glutamic acid and a method of producing L-glutamic acid using the same, and more specifically, to a novel nicotinamide mononucleotide transporter variant involved in the L-glutamic acid biosynthetic pathway, a polynucleotide, and a transformant, as well as a method of producing L-glutamic acid using the same. The nicotinamide mononucleotide transporter variant according to the present invention is obtained by substituting one or more amino acids in the amino acid sequence constituting nicotinamide mononucleotide transporter to change the enzymatic activity of the nicotinamide mononucleotide transporter, and a recombinant microorganism comprising the nicotinamide mononucleotide transporter variant is capable of efficiently producing L-glutamic acid.

The present disclosure provides a novel promoter variant in which some nucleotides are inserted, deleted, or substituted in a twin arginine translocase A (tatA) gene promoter of Escherichia coli . According to the present disclosure, the novel promoter variant can constitutively high-express a target protein, particularly an enzyme, in Escherichia coli . Therefore, by using a recombinant strain transformed with an expression vector including the novel promoter variant according to the present disclosure, it is possible to economically mass-produce a target protein, particularly an enzyme. For example, by using a recombinant strain transformed with an expression vector including the novel promoter variant according to the present disclosure, it is possible to economically mass-produce allulose epimerase or economically mass-produce allulose from fructose.

The present invention relates to a Corynebacterium glutamicum mutant strain having enhanced L-lysine productivity and a method of producing L-lysine using the same. The Corynebacterium glutamicum mutant strain is able to produce L-lysine in an improved yield as a result of improving the activity of glyceraldehyde 3-phosphate dehydrogenase by mutagenesis of amino acids in the gene encoding glyceraldehyde 3-phosphate dehydrogenase.

The present invention relates to an E. coli hisG-derived ATP-phosphoribosyltransferase variant having a reduced feedback inhibition by histidine and a strain expressing the same. The variant may maintain its activity even at a high histidine concentration, thus increasing histidine production.

The present invention relates to a Corynebacterium glutamicum mutant strain having enhanced L-lysine productivity and a method of producing L-lysine using the same. The Corynebacterium glutamicum mutant strain is able to produce L-lysine in an improved yield as a result of increasing the supply of the L-lysine precursor and sugar utilization by increasing or enhancing the expression of the gene encoding enolase and/or reducing or weakening the expression of the gluconate operon transcriptional repressor.

The present disclosure relates to a Corynebacterium glutamicum mutant strain having enhanced L-lysine productivity and a method of producing L-lysine using the same. The mutant strain may produce L-lysine in an improved yield compared to the parent strain by increasing or enhancing the expression of a gene encoding aspartate-semialdehyde dehydrogenase therein.