I PEACE, INC.

A cell culture vessel comprising a housing in which a culture chamber is provided. In the housing, at least two holes that connect the outside of the housing and a culture chamber are provided.

A microscope observation system includes a cell production device with a culture vessel in which cells or cell masses are cultured, a microscope device observing an object including cells or cell masses, a conveying apparatus conveying the microscope device to the cell production device, and an operating control section sending a command to the conveying apparatus to temporarily move the conveying apparatus from a current position to a relay position and then to move the conveying apparatus to a target position when focusing the microscope device onto the object. A first distance from the current position to the relay position is a distance necessary to drive an actuator by at least a predetermined amount in order to actuate a rotation shaft or a translation shaft of the conveying apparatus, and a second distance from the current position to the target position is a shorter distance than the first distance.

A microscope observation system comprises a cell production device provided with a culture vessel in which cells or cell masses are cultured, a microscope device capable of observing an object including cells or cell masses, a conveying apparatus that conveys the microscope device to the cell production device, and an operating control section that sends a command to the conveying apparatus to temporarily move the conveying apparatus from a current position to a relay position and then to move the conveying apparatus to a target position when focusing the microscope device onto the object, wherein a first distance from the current position to the relay position is a distance necessary to drive an actuator by at least a predetermined amount in order to actuate a rotation shaft or a translation shaft of the conveying apparatus, and a second distance from the current position to the target position is a shorter distance than the first distance.

[Problem] To provide cells that exhibit reduced immune rejection upon transplantation. [Solution] A cell that is homozygous for at least one, two, three, four, or all of the group consisting of HLA-A*01:01g, HLA-B*08:01g, HLA-DRB1*03:01, HLA-C*07:01g, HLA-DQB1*02:01g, and HLA-DRB3*01:01.

This cell treatment device is provided with: a factor introduction device 30 for introducing a pluripotent induction factor into cells so as to prepare induction factor-introduced cells; and a reprogramming suspension culture vessel for culturing the induction factor-introduced cells that have been prepared by the factor introduction device 30.

According to the present disclosure, a method for producing stem cells is provided, comprising applying tetrakis-pivaloyloxymethyl 2-(thiazol-2-ylamino) ethylidene-1, 1-bisphosphonate (PTA) to blood cells or immune cells, and inducing stem cells from said blood cells or immune cells.

Provided are a pharmaceutical composition or a pharmaceutical composition raw material, which contains supernatant from a culture medium used during culture to induce pluripotent stem cells.

Provided is a cell culture method including introducing a factor into cells in a cell culture vessel, and culturing the cells into which the factor has been introduced in the same cell culture vessel. Also provided is a mononuclear cell collection method including treating blood to prepare a treated blood from which erythrocytes have been at least partially removed, diluting the treated blood, causing sedimentation of mononuclear cells contained in the diluted treated blood, removing the supernatant from the diluted treated blood, and collecting the mononuclear cells.

This cell production system is provided with: a robot that assists cell production; and a plurality of closed system cell production devices which are affected by the robot in a one-to-many manner, wherein the closed system cell production devices are each provided with a double-sided structure of a dangerous region side affected by the robot and a safe region side on the reverse side of the dangerous region side.

This cell production system is provided with: a robot that assists cell production; and a plurality of closed system cell production devices which are affected by the robot in a one-to-many manner, wherein the closed system cell production devices are each provided with a double-sided structure of a dangerous region side affected by the robot and a safe region side on the reverse side of the dangerous region side.