MGI Tech Co., Ltd.

Provided are a fluid system, a fluid transport method, a gene sequencer, and a biochemical assay method. The fluid transport method includes the following steps: step a) of aspirating a first fluid from a fluid cartridge by using a pump valve assembly, so that the first fluid is input from an outlet of a flow cell into a lane of the flow cell, and the first fluid output from an inlet of the flow cell is returned to the fluid cartridge; and/or step b) of aspirating a second fluid from a fluid cartridge by using a pump valve assembly, so that the second fluid is input from the inlet of the flow cell into the lane of the flow cell, and the second fluid output from the outlet of the flow cell is returned to the fluid cartridge.

A chip processing device integrated with a reagent kit, a gene sequencer, a gene sequencing apparatus, and a method of performing biochemical detection. The chip processing device includes a substrate extending in a first direction, and a reagent kit platform and a chip platform assembled side-by-side and adjacently on the substrate in a second direction transverse to the first direction. The chip platform has a chip receiving area for accommodating a chip, the reagent kit platform has an accommodating chamber, and the reagent kit is received in the accommodating chamber. The reagent kit has a first fluid transport structure, the chip platform has a second fluid transport structure located on the chip platform and at least partially overlap and communicate with the first fluid transport structure. The first fluid transport structure is in fluid communication with the chip via the second fluid transport structure.

A method for analyzing droplets based on images: obtaining a working dye channel image and a reference dye channel image corresponding to a droplet system (S 1 ); separately performing a preset image processing operation on the working dye channel image and the reference dye channel image (S 2 ); using the working dye channel image or the reference dye channel image as a target image (S 3 ), and determining a region in which each droplet is located from within the target image (S 4 ); and on the basis of the determined region in which each droplet is located, analyzing the droplet system (S 5 ). The method can effectively improve the accuracy of droplet analysis. Further provided are a computer device and a storage medium.

An apparatus for forming a plurality of microdroplets ( 26 a, 26′, 56 ) from a droplet ( 16, 26, 263 ) includes a substrate ( 12, 22, 22 b, 22 c, 1101 ), a dielectric layer ( 13, 23, 23 b, 23 c, 43 a, 43 b, 43 ) on the substrate ( 12, 22, 22 b, 22 c, 1101 ) and having a plurality of hydrophilic surface regions ( 48 a, 491, 49 ) spaced apart from each other by a hydrophobic surface ( 44, 46 ), and a plurality of electrodes ( 14, 14 a, 14 b, 24, 24 a, 24 b, 24 c, 34 a, 34 b, 34 c ) in the dielectric layer ( 13, 23, 23 b, 23 c, 43 a, 43 b, 43 ). The electrodes ( 14, 14 a, 14 b, 24, 24 a, 24 b, 24 c, 34 a, 34 b, 34 c ) are configured to form an electric field (E) across the droplet ( 16, 26, 263 ) in response to voltages provided by a control circuit ( 15, 28, 67 ) to move the droplet ( 16, 26, 263 ) across the dielectric layer ( 13, 23, 23 b, 23 c, 43 a, 43 b, 43 ) in lateral direction (F) while leaving portions of the droplet ( 16, 26, 263 ) on the hydrophilic surface regions ( 48 a, 491, 49 ) to form the plurality of microdroplets ( 26 a, 26′, 56 ) on the hydrophilic surface regions ( 48 a, 491, 49 ).

The application discloses a flow cell system which includes a flow cell. The flow cell includes a substrate that is configured to support an analyte array, a cover, and an adhesive arrangement spacing the cover from the substrate to define a fluid gap between the substrate and cover. The flow cell also includes an inlet in fluid communication with the fluid gap and an outer perimeter. In one example implementation, fluid enters the flow cell at the inlet, flows through the fluid gap, and exits the flow cell at the outer perimeter between the substrate and the cover, with adhesive gaps in the adhesive arrangement at the outer perimeter of the flow cell allowing fluid to exit from the fluid gap.

A flow cell including a substrate that is configured to support an analyte array, a cover, and an adhesive arrangement spacing the cover from the substrate to define a fluid gap between the substrate and cover. The flow cell also includes an inlet in fluid communication with the fluid gap and an outer perimeter. In one example implementation, fluid enters the flow cell at the inlet, flows through the fluid gap, and exits the flow cell at the outer perimeter between the substrate and the cover; with adhesive gaps in the adhesive arrangement at the outer perimeter of the flow cell allowing fluid to exit from the fluid gap.

A flow cell includes a flow cell body. The flow cell body includes a frame and a fluid chamber defined in the flow cell body. The fluid chamber includes a reaction region allowing a fluid flow. A liquid inlet, a liquid outlet, and two exhaust holes connected to the fluid chamber are in the frame. Fluid into the liquid inlet flows through the reaction region in the fluid chamber and flows out through the liquid outlet. The exhaust holes discharge gas generated in the fluid chamber during the fluid flow. A flow cell with integral sealing rings and a biochemical substance reaction device are also disclosed.

A microfluidic apparatus ( 100 ) can include a PCB ( 110 ), a biological chip ( 120 ) overlying the PCB ( 110 ), and a microfluidic housing ( 130 ) overlying the biological chip ( 120 ) and the PCB ( 110 ). The microfluidic apparatus ( 100 ) also has a first adhesive layer ( 141 ) attaching the microfluidic housing ( 130 ) to the biological chip ( 120 ) and a second adhesive layer ( 142 ) attaching the microfluidic housing ( 130 ) to the PCB ( 110 ). The second adhesive layer ( 142 ) is thicker than the first adhesive layer ( 141 ). The first adhesive layer ( 141 ) comprises a first adhesive material, and the second adhesive layer ( 142 ) comprises a second adhesive material.

An apparatus ( 100 ) including multiple biological chips ( 110,120 ) includes a substrate ( 101 ), a first adhesive layer ( 134 ) disposed on the substrate ( 101 ), a first biological chip ( 110 ) and a second biological chip ( 120 ) disposed on the first adhesive layer ( 134 ) and attached to the substrate ( 101 ) by the adhesive layer ( 134 ). The apparatus ( 100 ) further includes a filler ( 130 ) disposed between the first biological chip ( 110 ) and the second biological chip ( 120 ). The filler ( 130 ) includes a second adhesive layer ( 135 ) extending between a side surface ( 114 ) of the first biological chip ( 110 ) and a side surface ( 124 ) of the second biological chip ( 120 ), the second adhesive layer ( 135 ) attaching the first biological chip ( 110 ) to the second biological chip ( 120 ). The filler ( 130 ) also includes a surface layer ( 132 ) disposed over the second adhesive layer ( 135 ). The surface layer ( 132 ) has a hydrophobic surface that is co-planar with a top surface ( 111 ) of the first biological chip ( 110 ) and a top surface ( 121 ) of the second biological chip ( 120 ).

A device for sorting cells includes a rotatable substrate ( 601 ) configured to rotate around a rotation axis ( 611 ) to generate a centrifugal force and a cover layer ( 441 ) attached to the rotatable substrate ( 601 ). The rotatable substrate ( 601 ) includes a sample reservoir ( 602 ) disposed at the center of the rotatable substrate ( 601 ), a flow path ( 604 ) coupled to the sample reservoir ( 602 ) and extending radially towards a periphery of the rotatable substrate ( 601 ) and including a bifurcation ( 606 ) into a main channel ( 607 ) and a side channel ( 608 ). The rotatable substrate ( 601 ) moves a plurality of cells stored in the sample reservoir ( 602 ) to a periphery of the rotatable substrate ( 601 ) by a centrifugal force. The cover layer ( 441 ) includes a first opening in fluid communication with the main channel ( 607 ) and a second opening in fluid communication with the side channel ( 608 ).