Nutcracker Therapeutics, Inc.

Apparatuses and methods process polynucleotides in a sealed path environment. The apparatuses include optical sensors to monitor operations and to track material usage for good manufacturing practice.

Microfluidic apparatuses (e.g., systems, devices, etc.) and methods for microfluidic provide detection of polynucleotide concentration. These apparatuses may include removable, single-use or reusable microfluidic members that may include one or more channels, chambers and/or substrates for performing microfluidic maneuvers and are adapted for detection of polynucleotide concentration. For example, the microfluidic member may be a microfluidic cartridge that may be inserted, held and/or seated in a microfluidic driver apparatus that may oversee and control operations within one or more cartridges based in part on the detected concentration of polynucleotide.

Provided herein are compositions and methods for identifying effective delivery vehicles (DVs) for mRNA delivery to and expression in specific cells or tissues in culture (in vitro) or in live animals (in vivo) from a pool of co-administered DVs.

Human SIRPa fusion proteins having an enhanced affinity to CD47 via increased binding valency. The human SIR-Pa fusion proteins described herein may form tetramer, hexamer, octamers, etc. These fusion proteins may be configured to form heterodimers with other fusion proteins, including C-C chemokine receptor type 4 (CCR4) binding fusion proteins.

A customized codon sequence may be generated using a method which comprises receiving a target amino acid sequence, generating a plurality of candidate codon sequences, and selecting, from a set of final codon sequences which comprises candidate codon sequences, a customized codon sequence. In such a method, each of the candidate codon sequences may be a codon sequence which codes for the target amino acid sequence, and the final codon sequences may be generated based on a set of initial codon sequences. Additionally, the final codon sequences may be organized into sets of final codon sequences, each of which sets corresponds to a vector from a set of vectors and may comprise codon sequences which are farther from an origin than typical codon sequence from a set of initial codon sequences. Corresponding systems and computer readable mediums for generating customized codon sequences may also be implemented.

The present disclosure provides delivery vehicle compositions comprising hydroxyalkyl-capped cationic peptoids, such as 2-aminopropane-1,3-diol-capped cationic peptoids, and complexes of the delivery vehicles with polyanionic compounds, such as nucleic acids. The disclosure further provides methods of making and using the delivery vehicle compositions and complexes, such as for the delivery polyanionic compounds (e.g., nucleic acids) to cells. The disclosure also provides methods of eliciting an immune response with the delivery vehicle complexes of the disclosure.

Apparatuses and methods are described herein for processing polynucleotides in a scaled path environment. The apparatuses include optical sensors to monitor operations and to track material usage for good manufacturing practice.

A microfluidic apparatus includes a first plate, a second plate, and a microfluidic path defined between the first plate and the second plate. The microfluidic path includes at least one chamber. The microfluidic apparatus also includes an elastic layer disposed between the first plate and the second plate. The elastic layer includes a first membrane extending across the at least one chamber. The first membrane is configured to deflect to drive a fluid through the at least one chamber. The elastic layer also includes a second membrane having at least one aperture. The at least one aperture is aligned with the at least one chamber.

Microfluidic apparatuses (e.g., systems, devices, etc.) and methods for microfluidic provide detection of polynucleotide concentration. These apparatuses may include removable, single-use or reusable microfluidic members that may include one or more channels, chambers and/or substrates for performing microfluidic maneuvers and are adapted for detection of polynucleotide concentration. For example, the microfluidic member may be a microfluidic cartridge that may be inserted, held and/or seated in a microfluidic driver apparatus that may oversee and control operations within one or more cartridges based in part on the detected concentration of polynucleotide.

Apparatuses and methods are described herein for processing polynucleotides in a sealed path environment. The apparatuses include optical sensors to monitor operations and to track material usage for good manufacturing practice.