Twist Bioscience Corporation

Provided herein are compositions and methods for improving hybridization reactions. Further provided herein are synthetic blocking libraries. Further provided herein are methods for designing synthetic blocking libraries, and application towards methylome analysis.

Provided herein are methods, systems, and compositions for efficient nucleic acid assembly with improved representation and distribution. Provided herein are methods, systems, and compositions for efficient nucleic acid assembly of nucleic acids encoding genes for use in various downstream processes.

Described herein are methods for polynucleotide synthesis. In some aspects, the methods may provide for synthesis of DNA sequences having full length sequences of 100 to 350 bases in length. In some aspects, the methods may provide for a synthesis rate of 10 to 25 nucleotides per hour. In some aspects, the methods may include receiving first instructions in a computer readable non-transient medium, processing the first instructions, and transmitting second instructions to a device for synthesis of polynucleotides.

Provided herein are compositions, methods and systems relating to libraries of polynucleotides having preselected stoichiometry with regard to species of polynucleotides such that the libraries allow for predetermined application outcomes, e.g., controlled representation after amplification and uniform enrichment after binding to target sequences. Further provided herein are polynucleotide probes and applications thereof for uniform and accurate next generation sequencing.

Systems, methods, and compositions for identifying genomic variants and methylation analysis, including synthetic polynucleotide libraries, are provided. The synthetic polynucleotide libraries may comprise a plurality of polynucleotides. The polynucleotides may comprise sequences corresponding to a genetic abnormality in a genome. The stoichiometry of each of the plurality of polynucleotides is controlled. Systems, methods, and compositions described herein may include standards for determining the analytical sensitivity and/or accuracy of instruments configured to measure nucleic acid variant frequencies. Standards may comprise RNA-fusions and/or CNV mutations related to cancer.

Provided herein are methods and compositions relating to glycan libraries having nucleic acids encoding for a scaffold comprising a glycan domain. Glycan libraries described herein encode for immunoglobulins such as antibodies.

Provided herein are compositions, devices, systems and methods for electrochemical synthesis. Further provided are devices comprising addressable electrodes controlling polynucleotide synthesis (deprotection, extension, or cleavage, etc.) The compositions, devices, systems and methods described herein provide improved synthesis, storage, density, and retrieval of biomolecule-based information.

Provided herein are compositions, methods and systems relating to libraries of polynucleotides such that the libraries allow for accurate and efficient hybridization after binding to target sequences. Further provided herein are probes, blockers, additives, buffers, and methods that result in improved hybridization. Such compositions and methods are useful for improvement of Next Generation Sequencing applications, such as reducing off-target binding or reducing workflow times.

Provided herein are antibodies and antibody fragments relating to SIGLEC-8 and CD117. Provided herein are methods and compositions relating to SIGLEC-8 and/or CD117 libraries having nucleic acids encoding for a scaffold comprising a SIGLEC-8 and/or CD117 domain. SIGLEC-8 and/or CD117 libraries described herein encode for immunoglobulins such as antibodies.

Disclosed herein are methods and compositions for cleavage of nucleic acids from a surface of a solid support. Further described herein are cleavage methods compatible with enzymatic and chemical nucleic acid synthesis methods.