Upside Foods, Inc.

Provided herein are methods of making a comestible cell-based meat composition where the method includes cooling the cell mass from a growth temperature to a temperature below a growth temperature; harvesting at least a portion of the cell mass; de-wetting the harvested cell mass with mechanically-generated pressure; forming the cell-based meat composition into a fillet; and optionally processing the fillet under high pressure, until the comestible cell-based meat composition satisfies a texture profile criteria.

This disclosure describes methods of forming an extruded cell-based food product that mimics the texture and flavor of slaughtered meat. Generally, the disclosed method comprises cultivating non-human animal cells. The disclosed methods combine the non-human cultivated animal cells with one or more plant proteins. In some embodiments, the disclosed methods also add one or more amino acids and/or a marinade to the non-human cultivated animal cells and the one or more plant proteins. In some embodiments, the disclosed methods extrude the non-human cultivated animal cells and one or more plant proteins utilizing high-moisture extrusion to form an extruded cell-based food product with a fibrous texture.

This disclosure describes methods of forming an extruded cell-based food product that mimics the texture and flavor of slaughtered meat. Generally, the disclosed method comprises cultivating non-human animal cells. The disclosed methods combine the non-human cultivated animal cells with one or more plant proteins. In some embodiments, the disclosed methods also add one or more amino acids and/or a marinade to the non-human cultivated animal cells and the one or more plant proteins. In some embodiments, the disclosed methods extrude the non-human cultivated animal cells and one or more plant proteins utilizing high-moisture extrusion to form an extruded cell-based food product with a fibrous texture.

This disclosure generally describes comestible cell-based food products including a combination of cultured cells and dry cell powder and methods for preparing such cell-based food products. In one or more embodiments, the comestible cell-based food product comprises a mixture of cultured animal cells and dry cultured animal cell powder. For example, the comestible cell-based food product is produced by generating a homogenous mixture of cultured animal cells and dry cultured animal cell powder. In one or more embodiments, the dry cultured animal cell powder improves the texture of the resultant comestible cell-based food product by tailoring the hardness and/or adhesiveness of the comestible cell-based food product.

The apparatuses described herein relate to preparation of a meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.

Provided herein are methods of making a comestible cell-based meat composition where the method includes cooling the cell mass from a growth temperature to a temperature below a growth temperature; harvesting at least a portion of the cell mass; de-wetting the harvested cell mass with mechanically-generated pressure; forming the cell-based meat composition into a fillet; and optionally processing the fillet under high pressure, until the comestible cell-based meat composition satisfies a texture profile criteria.

The apparatuses described herein relate to preparation of a meat product. Apparatuses, systems comprising the apparatuses, and methods of making and use the systems and apparatuses are described herein. These are useful for controlling one or more of growth on and separation of a meat product from an enclosed substrate. The apparatuses and systems are configured to receive fluid and grow the meat product and/or separate the meat product from the substrate in a scalable manner.

Provided herein are cell-based meat compositions suitable for consumption comprising cells harvested from suspension cell culture and comprising an emulsion mixture. Also provide here are methods of making a cell-based meat composition suitable for consumption comprising: culturing a population of cells in suspension; harvesting the population of cells to produce a cell mass; formulating an emulsion mixture by: (i) emulsifying an oil with a hydrocolloid to produce an oil:hydrocolloid mixture; and (ii) adding the cell mass to the oil:hydrocolloid mixture to form an emulsion mixture; and (d) forming the cell-based meat composition into a patty.

This disclosure relates to methods of shaping a raw cell-based-meat product using vacuum sealing. The disclosed method includes preparing a cell-mass mixture by mixing a grown cell mass with a binding agent. The cell-mass mixture is added to a mold that is covered with a film and vacuum sealed. Because, in some embodiments, the mold is rigid, the cell-mass mixture conforms to the shape of the mold, such as the shape of slaughtered or harvested meat. To shape a cell-based-meat product, an apparatus may be used with a mold for sealing and conforming a cell-mass mixture.

The present disclosure provides methods and compositions useful for the production of slaughter-free meat products, and the characterizations of the same. The slaughter-free meat products contain several points of distinction when compared to conventional meat procured by harvesting the tissue of a dead animal. Such points of distinction include, but are not limited to, significantly reduced or substantially no: steroid hormones, antibiotics, or microbial contamination; lower fat content; no vasculature; and extended shelf life both at room temperature and when refrigerated.