WAKE FOREST UNIVERSITY HEALTH SCIENCES

Methods and compositions are disclosed for repair of shoulder injuries by employing disaggregated muscle fiber fragments to regenerate functional shoulder muscle tissue. In some embodiments, the fragments retain functional satellite cells but exhibit cell wall rupture and have an average size of less than 150 μm. The methods include the preparation and implantation of compositions by extracting muscle tissue from a donor site, disaggregating muscle fibers from the extracted tissue, and fragmenting disaggregated muscle fibers into fiber fragments that exhibit cell wall rupture and preferably have an average size of less than 150 microns, more preferable less than about 100 microns, while retaining functional satellite cells. Upon injection, e.g., into the supraspinatus or other rotator cuff muscles, the muscle fiber fragment compositions are capable of reconstituting or reconstructing elongated muscle fibers from the fragments and orienting in alignment with native shoulder muscle fibers.

Provided herein according to embodiments is a composition comprising polysaccharides isolated from sago or acorn for use as a dietary supplement or food additive. The composition is useful to improve gut health in a subject. Methods of extracting the polysaccharides and food products comprising the same are also provided.

Disclosed herein are method of treating hemophilia A in a subject comprising injecting the subject with mesenchymal stromal/stem cells (MSC) modified to express high levels of Factor VIII protein. The MSC are isolated prenatally, at birth, or after the subject's birth. The modified MSC may also express high levels von Willebrand factor protein.

This invention relates engineered extracellular vesicles (EVs) comprising a membrane-bound interleukin-2 (IL-2) molecule and/or functional fragment thereof, a major histocompatibility (MHC) molecule and/or functional fragment thereof, and one or more costimulatory molecule, each expressed on the surface membrane of the EV. The invention further relates to compositions, cells and kits comprising the same, and methods of using and making the same.

Provided according to some embodiments is a compound of Formula (I), or a pharmaceutically acceptable salt thereof. Pharmaceutical compositions formulated for oral or parenteral administration comprising the same and methods of use for treating cancer and inhibiting PRX3 comprising administering to the subject a therapeutically effective amount of a compound are also provided.

A method of providing a culture of oogonia stem cells comprising oogonia stem cells is provided. The method may further include culturing the oogonia stem cells with granulosa and theca cells to differentiate the oogonia stem cells into oocytes. In some embodiments, the culturing comprises including the oogonia stem cells in an in vitro follicle construct or a microcapsule comprising said granulosa and theca cells. Further described herein is a method of forming a bioengineered follicle construct capable of releasing a mature oocyte. An in vitro fertilization method using the mature oocyte is also provided.

The present invention is directed to radiopharmaceuticals with improved stability, a kit, and a method of production thereof.

Methods and compositions are disclosed for repair of shoulder injuries by employing disaggregated muscle fiber fragments to regenerate functional shoulder muscle tissue. In some embodiments, the fragments retain functional satellite cells but exhibit cell wall rupture and have an average size of less than 150 μm. The methods include the preparation and implantation of compositions by extracting muscle tissue from a donor site, disaggregating muscle fibers from the extracted tissue, and fragmenting disaggregated muscle fibers into fiber fragments that exhibit cell wall rupture and preferably have an average size of less than 150 microns, more preferable less than about 100 microns, while retaining functional satellite cells. Upon injection, e.g., into the supraspinatus or other rotator cuff muscles, the muscle fiber fragment compositions are capable of reconstituting or reconstructing elongated muscle fibers from the fragments and orienting in alignment with native shoulder muscle fibers.

Provided herein according to embodiments is a composition comprising polysaccharides isolated from sago or acorn for use as a dietary supplement or food additive. The composition is useful to improve gut health in a subject. Methods of extracting the polysaccharides and food products comprising the same are also provided.

Provided according to some embodiments is a compound of Formula (1), a 2-aryl, 2-heteroaryl, 2-cycloalkyl or 2-heterocycle substituted thiazole-4-carboxamido) acrylamido)acrylate compound, as a peroxiredoxin 3 (PRX3) inhibitor, or a pharmaceutically acceptable salt or prodrug thereof. Pharmaceutical compositions comprising the same and methods of use for treating cancer and inhibiting PRX3 in a subject in need thereof, are also provided.