MGI Tech Co., Ltd.

The present invention falls within the field of biotechnology, and specifically discloses a method for constructing a cyclized library and a ring-forming linker. The method comprises: 1) breaking a DNA sequence into fragments; 2) enabling two ends of the broken fragment to form 3′ end protrusions; and 3) cyclizing the fragment with 3′ end protrusions to form a ring-shaped library by means of a ring-forming linker, wherein the ring-forming linker is a double chain which is not completely paired and has 3′ end protrusion at both ends, and the 3′ end protrusion of the ring-forming linker is complementary to the 3′ end protrusion of the broken fragment. According to the present invention, an A-sticky end of the end is formed by means of repairing the end of and the adding A to the broken DNA fragment, and same is then complementary to the specifically designed linker T sticky end to form a cyclized structure, and the connection at the gap is completed under the action of a ligase.

An apparatus for forming a plurality of microdroplets from a droplet includes a substrate, a dielectric layer on the substrate and having a plurality of hydrophilic surface regions spaced apart from each other by a hydrophobic surface, and a plurality of electrodes covered by the dielectric layer. The electrodes are configured to form an electric field across the droplet in response to voltages provided by a control circuit to move the droplet across the dielectric layer in a lateral direction while leaving portions of the droplet on the hydrophilic surface regions to form the plurality of microdroplets on the hydrophilic surface regions.

A device for sorting cells includes a rotatable substrate ( 601 ) configured to rotate around a rotation axis ( 611 ) to generate a centrifugal force and a cover layer ( 441 ) attached to the rotatable substrate ( 601 ). The rotatable substrate ( 601 ) includes a sample reservoir ( 602 ) disposed at the center of the rotatable substrate ( 601 ), a flow path ( 604 ) coupled to the sample reservoir ( 602 ) and extending radially towards a periphery of the rotatable substrate ( 601 ) and including a bifurcation ( 606 ) into a main channel ( 607 ) and a side channel ( 608 ). The rotatable substrate ( 601 ) moves a plurality of cells stored in the sample reservoir ( 602 ) to a periphery of the rotatable substrate ( 601 ) by a centrifugal force. The cover layer ( 441 ) includes a first opening in fluid communication with the main channel ( 607 ) and a second opening in fluid communication with the side channel ( 608 ).

Disclosed herein are DNA sequencing methods involving applying a long DNA molecule to an indexed array comprising an array of transfer sites (TS). Each TS comprises a substrate and a source of clonal barcodes (SCB) attached to or situated on the substrate. Each SCB comprises many copies of a unique transferable barcode sequence, and the unique transferable barcode sequence associated with each TS is known. The unique transferable barcode sequence is transferred from the SCB portion of the TS to a location on the long DNA molecule. DNA fragments comprising the barcode sequences are recovered from the array and sequenced. Sequence reads from these fragments are assembled based on the relative positions of the TS on the array.

A liquid transfer device for a liquid transfer with a sample carrier of a reaction platform is provided and includes a substrate, a driving device, and a control device. The control device is used to control the driving device to drive the substrate to move towards the reaction platform. Such that the moving substrate passes through the sample carrier and transfers a liquid with the sample carrier. The liquid transfer refers to a transfer of a liquid carried by the substrate to the sample carrier and/or a transfer of the liquid on the sample carrier to the substrate. A liquid transfer method, a biochemical substance reaction device, a biochemical substance analysis device, and a biochemical substance analysis method are disclosed. A throughput of a biochemical reaction and analysis is improved, and lower the cost.

An apparatus for forming a plurality of microdroplets from a droplet includes a substrate, a dielectric layer on the substrate and having a plurality of hydrophilic surface regions spaced apart from each other by a hydrophobic surface, and a plurality of electrodes covered by the dielectric layer. The electrodes are configured to form an electric field across the droplet in response to voltages provided by a control circuit to move the droplet across the dielectric layer in a lateral direction while leaving portions of the droplet on the hydrophilic surface regions to form the plurality of microdroplets on the hydrophilic surface regions.

A gene sequencing reaction device, a gene sequencing system and a gene sequencing reaction method. The gene sequencing reaction device includes: a supporting platform; a dipping container disposed on the supporting platform, wherein the dipping container has a dipping reaction area, and the dipping reaction area is configured to hold a chemical reagent for gene sequencing reaction, so as to dip a sequencing chip having a DNA sample loading structure on the surface and having a DNA sample loaded thereon in the chemical reagent to perform a gene sequencing reaction; a temperature control apparatus, configured to control the temperature of the chemical reagent in the dipping reaction area; and a transfer apparatus, configured to insert the sequencing chip into the dipping reaction area or pull out the sequencing chip from the dipping reaction area.

A test system comprising: a carrier disc configured to carry a target sample; a light source module configured for emitting at least two types of reference light with different wavelengths; a light guiding module located on an emitting path of the reference light and configured for guiding the reference light to the target sample and for receiving and guiding at least two types of detection light with different wavelengths, the detection light being generated by the target sample according to the reference light; and a detection module configured for receiving the detection light and obtaining biochemical information of the target sample according to the detection light.

Proposed by the present disclosure are an FPGA-based resequencing analysis method and device, wherein the method comprises: receiving genomic resequencing data; using the resequencing data as an input of an FPGA, determining a comparison result in the resequencing process according to an output of the FPGA, and simultaneously performing sorting and deduplication processing on the comparison result; correcting a base quality value of the comparison result after sorting and deduplication processing; and detecting a mutation result according to the corrected comparison result. The described method may save program running time, save calculation costs, and improve resequencing efficiency.

A nucleic acid sequencing system may include non-planar substrates coupled to the outer surface of a rotating drum. The substrates may be curved and include a plurality of nucleic acid samples. A detection system, including for example an objective and a camera, may detect sequencing events on the non-planar substrate while the non-planar substrate is rotated relative to the detection system around a longitudinal axis of the drum by the actuation system.